DSIP (Delta Sleep-Inducing Peptide): Sequence, Structure & Technical Data
DSIP (delta sleep-inducing peptide, CAS 62568-57-4) is a nine-amino-acid neuropeptide, Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. It was first isolated in the 1970s and named for its reported effect on slow-wave (“delta”) brain activity in rabbits. Decades later, its natural receptor and gene are still unknown, which makes it an unusual open question in neuropeptide research. This page is a laboratory reference covering its sequence, molecular data, research background, handling, storage and testing. For research use only. Not for human or veterinary use.
| Compound | DSIP (delta sleep-inducing peptide; INN emideltide) |
| CAS number | 62568-57-4 |
| Sequence | Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu (WAGGDASGE) |
| Molecular formula | C35H48N10O15 |
| Molecular weight | ≈ 848.8 g/mol |
| Length | 9 amino acids, linear |
| Form supplied | Lyophilized powder |
| Purity specification | ≥ 99% by HPLC, identity by mass spectrometry, lot-matched COA |
What is DSIP?
In 1977, Schoenenberger and Monnier in Basel reported a peptide isolated from the blood of rabbits kept in an induced sleep state. When given to other rabbits, it was reported to increase slow-wave (delta) EEG activity, so they called it delta sleep-inducing peptide (Schoenenberger and Monnier, PNAS, 1977).
Later research produced mixed results. Some studies found DSIP-like activity in the brain, pituitary and gut and reported effects on stress hormones, temperature regulation and other systems, while others could not reproduce consistent sleep effects. A 2006 review described DSIP as “a still unresolved riddle”, because no dedicated gene, precursor protein or receptor has been identified (Kovalzon and Strekalova, Journal of Neurochemistry, 2006).
DSIP is not an approved medicine. Research-grade DSIP is a laboratory reagent.
Structure and sequence
- Tryptophan at the N-terminus gives the peptide UV absorbance and fluorescence, which is useful for detection in the lab.
- Two acidic residues (Asp5 and Glu9) give DSIP an overall negative charge at neutral pH.
- Ser7 has been reported as a phosphorylation site; a phosphorylated form (P-DSIP) has been described in the literature.
- Gly- and Ala-rich backbone. Small residues make the chain flexible, and in solution DSIP has no fixed folded structure.
Research background
- Sleep EEG. The founding studies measured delta-wave activity in rabbits; follow-up animal work gave inconsistent results across species and methods.
- Stress and neuroendocrine systems. Animal studies have examined DSIP in relation to stress-hormone release and adaptation to stress.
- Unknown receptor. Without a confirmed receptor or gene, DSIP is often used in research asking how such short peptides act, and whether DSIP-like immunoreactivity comes from a larger, still-unidentified protein (Kovalzon and Strekalova, 2006).
Animal and cell results describe biology in specific models and cannot be assumed to translate directly to people.
Reconstitution and handling (laboratory)
- Let the sealed vial reach room temperature before opening.
- DSIP is water-soluble. Reconstitute with sterile water, sterile water or a buffer suited to your assay.
- Short, unstructured peptides like DSIP are quickly broken down by peptidases in biological samples; plan sampling times and inhibitors accordingly.
- Aliquot and freeze to avoid repeated freeze-thaw.
See the Reconstitution and Storage guide.
Storage and stability
- Lyophilized powder: sealed at −20 °C, protected from light and moisture.
- In solution: 2–8 °C short term, or aliquots frozen at −20 °C or below.
How Zama DSIP is tested
- HPLC purity (≥ 99% specification) and mass spectrometry identity against the expected mass of about 848.8 Da. See HPLC vs Mass Spectrometry.
- Lot-matched COA in the COA Library.
Frequently asked questions
What is DSIP?
Delta sleep-inducing peptide, a nine-residue neuropeptide (WAGGDASGE) first isolated in 1977 and studied in sleep and neuroendocrine research.
What is the CAS number of DSIP?
62568-57-4. Its molecular formula is C35H48N10O15 and its molecular weight is about 848.8 g/mol.
Does DSIP have a known receptor?
No dedicated receptor or gene has been confirmed, which is why reviews describe it as unresolved.
Is DSIP approved?
No. DSIP is a research compound and not for human or veterinary use.
Researcher access
DSIP is available to registered researchers. Sign in or create your researcher account to view DSIP →
Related research guides: Selank · Semax · Epithalon · Full Research Library
References
- Schoenenberger GA, Monnier M. Proceedings of the National Academy of Sciences USA. 1977;74(3):1282–1286.
- Kovalzon VM, Strekalova TV. Journal of Neurochemistry. 2006;97(2):303–309.
Research Use Only. DSIP and all products referenced are sold strictly for in-vitro laboratory research. They are not drugs, supplements or medical devices, and are not for human or veterinary use. This page summarizes published literature and makes no claim about any product. Statements have not been evaluated by the FDA.